Anew tetrahydrofuran-type lignan from leaves of Magnolia denudata / 中草药

Objective: To study the lignans in the leaves of Magnolia denudata. Methods: The constituents were separated and purified by silica gel, Sephadex LH-20 and semi-preparative HPLC. The structures were elucidated on the basis of spectroscopic data analysis. All compounds were evaluated for their inhibitory activity against LPS-activated NO production in RAW 264.7 cell model in vitro. Results: Two tetrahydrofuran-type lignans and three dihydrobenzofuran-type lignans were isolated from 95% ethanol extract of M. denudate. Their structures were identified as (7S,7'R,8S,8'S)-3,4,5-trimethoxy-3',4'-methylenedioxy-7,7'-epoxylignan (1), veraguensin (2), liliflol A (3), liliflol B (4) and 5-methoxyliliflol B (5). Conclusion: Compound 1 is a new compound and named as magnodatin A. Compound 5 is a new natural product. The 13C-NMR spectroscopic data of compounds 3-5 are reported for the first time. Compound 5 exhibited weak inhibitory effect on NO production in LPS-induced macrophages with a inhibiton rate of 48.7% at 50 μmol/L.

Simultaneous Determination of Seven Residual Solvents in Nilethylenol Raw Material by GC / 中国药房

OBJECTIVE： To establish a method for simultaneous determination of residual methanol， ethanol， acetonitrile， isopropanol， methylbenzene， tetrahydrofuran and ethyl acetate in Nilethylenol raw material. METHODS： GC was performed. The determination was performed on DB-WAX capillary column. The detector was hydrogen flame ionization detector with split ratio of 5 ∶ 1. The carrier gas was nitrogen （purity： 99.999％） at the flow rate of 1.0 mL/min. The sample size was 1 μL， directly sampling with bonded crosslinked polyethylene glycol as stationary phase. The initial temperature was 40 ℃ and was maintained for 5 min， increased to 90 ℃ at 10 ℃/min， and then increased to 200 ℃ at 5 ℃/min. The temperature of injector was 220 ℃， and detector temperature was 230 ℃. RESULTS： The linear range was 0.24-12.00 μg/mL for methanol （r＝0.999 7）， 0.40-20.00 μg/mL for ethanol （r＝0.999 5）， 0.033-1.64 μg/mL for acetonitrile （r＝0.999 8）， 0.40-20.00 μg/mL for isopropanol （r＝0.999 5）， 0.071-3.56 μg/mL for methylbenzene （r＝0.999 6）， 0.058-2.88 μg/mL for tetrahydrofuran （r＝0.999 8）， 0.40-20.00 μg/mL for ethyl acetate （r＝0.999 7）， respectively. The limits of quantitation were 0.24， 0.40， 0.033， 0.40， 0.071， 0.058， 0.40 μg/mL， respectively. The limits of detection were 0.08， 0.10， 0.01， 0.13， 0.02， 0.02， 0.13 μg/mL， respectively. RSDs of precision， stability and reproducibility tests were all lower than 2％. The recoveries were 98.17％-100.48％ （RSD＝0.92％，n＝9）， 97.77％-101.30％（RSD＝1.32％，n＝9）， 97.56％-100.85％（RSD＝1.20％，n＝9）， 98.64％-100.92％（RSD＝0.87％，n＝9）， 98.54％-100.62％（RSD＝0.76％，n＝9），98.26％-100.00％（RSD＝0.74％，n＝9）， 98.30％-100.59％（RSD＝0.76％，n＝9）， respectively. CONCLUSIONS： The method has high sensitivity and good accuracy， and can be used for the simultaneous determination of residual methanol， ethanol， acetonitrile， isopropane， methylbenzene， tetrahydrofuran and ethyl acetate in Nilethylenol raw material.

Secondary metabolites from Colletotrichum fioriniae F18, an endophytic fungus isolated from the medicinal plant Mahonia fortunei / 药学学报

Chemical investigation on the rice culture of an endophytic fungus Colletotrichum fioriniae F18, inhabiting in the stems of the medicinal plant Mahonia fortunei, led to the isolation of nine compounds. They included a new indole alkaloid, makomotindoline B (1), and two known indole derivatives, 3-indoleacetic acid methyl ester (2) and N-acetyltryptamine (3), together with six known aromatic compounds, 2-(4-hydroxyphenyl) acetic acid (4), 4-(2-hydroxyethyl)phenol (5), 2-(4-methoxyphenyl)acetic acid (6), 4-hydroxyphenethyl 2-(4-hydroxyphenyl)acetate (7), regiolone (8) and N-phenethylacetamide (9). The structures of these compounds were elucidated based on the analysis of spectroscopic data including MS and NMR. The absolute configuretion of compound 1 was determined by electronic circular dichroism (ECD) calculation. Antibacterial activity assay indicated that compounds 1-9 had no antibacterial activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as no quorum sensing inhibitory (QSI) activity for Chromobacterium violaceum.

Chemical constituents from ethyl acetate extract of Gleditsiae Spina / 中草药

Objective To investigate the chemical constituents from the ethyl acetate extract of Gleditsiae Spina. Methods The chemical constituents were isolated and identified by chromatography on silica gel, Toyopearl HW-40C, Sephadex LH-20, MCI Gel CHP-20, ODS, and RP-HPLC. Their structures were elucidated on the basis of physicochemical properties and spectral analyses. Results Eighteen compounds were isolated from the ethyl acetate extract of Gleditsiae Spina, and identified as scopoletin (1), (-)-(7R,8S)-erythro-guaiacylglycerol (2), 5-methoxy-guaiacylglycerol (3), (-)-(7R,8R)-threo-guaiacylglycerol (4), 3,4’- dihydroxypropiophenone (5), dihydroferulic acid (6), p-hydroxybenzoic acid (7), indole-3-carbaldehyde (8), protocatechuic acid methyl (9), protocatechuic aldehyde (10), syringic acid (11), 2-guaiacylpropane-1,3-diol (12), (2R*,3R*,4S*)-2,3-diguaiacyl-4- hydroxyl tetrahydrofuran (13), 3-(2-oxopropyl)-3-hydroxy-indolin-2-one (14), juglanin D (15), C-veratroylglycol (16), 3-(4-hydroxyl-3-methoxyphenyl)-propan-1,2-diol (17), and (-)-syringaresinol (18). Conclusion Eighteen compounds are isolated from this plant for the first time.

A new tetrahydrofuran lignan from Peperomia blanda / 国际药学研究杂志

Objective To investigate the chemical constituents of Peperomia blanda. Methods Guided by HPLC detection, integrated methods including vacuum liquid chromatography (VLC), ODS and semi-preparative RP-HPLC were used for separation. The structure was determined by spectral analyses including ESI-MS, 1D NMR, 2D NMR and ECD spectra. Results Four compounds were isolated and identified as (7S,7’S,8R,8 ’R)-7-(5-methoxy-3,4-methylenedioxyphenyl)-7-(4-hydroxy-3,5-dimethoxy-phenyl)-8, 8-dihydroxymethyltetrahydrofuran (1), 7, 8-trans-8,8-trans-7,8-cis-7, 7-(4-hydroxy-3,5-dimethoxy phenyl)-8,8-di-acetoxymethyltetrahydrofuran (2), (+)-(7S, 7S, 8R, 8R)-4, 4-dihydroxy-3, 3, 5, 5-tetramethoxy-7, 9, 7, 9-diepoxylignane (3), and (6R, 7E, 9R)-9-hydroxy-4, 7-megastigmadien-3-one (4). Conclusion Compound 1 is a new tetrahydrofuran lignan, compounds 2-4 were isolated from P. blanda for the first time.

Testing tetrahydrofuran in the air of workplace by solvent desorption-gas chromatography / 中国职业医学

OBJECTIVE: To establish a method for testing tetrahydrofuran( THF) in workplace air by solvent desorption gas chromatography. METHODS: The air samples were collected by activated carbon tube,desorbed with carbon disulfide solution,then separated by capillary column and detected by flame ionization detector. RESULTS: The linearity range of THF concentration was 1. 78-8 892. 00 mg / L,and the correlation coefficient was 0. 999 9. The minimum detection limit of THF was 0. 09 mg / m3. The minimum quantitative concentration was 0. 27 mg / m3( 3. 00 L air collection). The average desorption efficiency of THF was 94. 29%-96. 46% when placed overnight at the room temperature. The within-run and the between-run relative standard deviation were 0. 30%-0. 91% and 0. 66%-1. 23% respectively. THF sample could be stored at room temperature for at least 8 days. CONCLUSION: The method could be widely applied in sampling and detection of THF in workplace air.

A new tetrahydrofuran lignan from Peperomia blanda / 国际药学研究杂志

Objective To investigate the chemical constituents of Peperomia blanda. Methods Guided by HPLC detec?tion,integrated methods including vacuum liquid chromatography(VLC),ODS and semi-preparative RP-HPLC were used for separa?tion. The structure was determined by spectral analyses including ESI-MS,1D NMR,2D NMR and ECD spectra. Results Four com?pounds were isolated and identified as(7S,7′S,8R,8′R)-7-(5-methoxy-3,4-methylenedioxyphenyl)-7′-(4-hydroxy-3,5-dimethoxy?phenyl)-8,8′-dihydroxymethyltetrahydrofuran(1),7,8-trans-8,8′-trans-7′,8′-cis-7,7′-(4-hydroxy-3,5-dimethoxy phenyl)-8,8′-di?acetoxymethyltetrahydrofuran(2),(+)-(7S,7′S,8R,8′R)-4,4′-dihydroxy-3,3′,5,5′-tetramethoxy-7,9′,7′,9-diepoxylignane(3), and(6R,7E,9R)-9-hydroxy-4,7-megastigmadien-3-one(4). Conclusion Compound 1 is a new tetrahydrofuran lignan,compounds 2-4 were isolated from P. blanda for the first time.

Assessment of biodegradability of PVC containing cellulose by white rot fungus

Aims: Polyvinyl chloride (PVC) is the most widely used and environmentally damaging plastic. Processing, production and disposal of PVC cause release of toxic chlorine based compounds into environment. The objective of the present study was to assess the biodegradability of cellulose blended PVC by white rot fungi i.e. Phanerochaete chrysosporium. Methodology and results: Biodegradability of the strain for the polymer was tested on plate assay, sturm test, soil burial and shake flask experiments. The biodegradability of the polymer was determined by visual changes, plate assay and carbon dioxide production. Morphological changes in the polymer such as pits, extensive spotting, clear surface erosion, fungal attachment, roughening and deterioration of some parts were observed using scanning electron microscopy. Chemical changes like appearance and shortening of peaks using fourier transform infrared spectroscopy also confirmed the biodegradability of the polymer. Conclusion, significance and impact of study: The present study confirmed that mixing of small amount of cellulose increases the hydrophilicity of the polymer and lead to its microbial degradation and Phanerochaete chrysosporium has great potential for the treatment of solid waste containing plastics.